1. The movement of molecules from a region of higher concentration to lower concentration is called ____________.
A) Osmosis
B) Diffusion
C) Active transport
D) Imbibition
Answer: B
Explanation: Diffusion is a passive movement of molecules along the concentration gradient.
2. The movement of water through a semi-permeable membrane is called ____________.
A) Diffusion
B) Osmosis
C) Imbibition
D) Transpiration
Answer: B
Explanation: Osmosis is the passive diffusion of water molecules across a semipermeable membrane.
3. Imbibition is important in ____________.
A) Root elongation
B) Seed germination
C) Photosynthesis
D) Transpiration
Answer: B
Explanation: Dry seeds imbibe water due to hydrophilic colloids in seed coats, initiating germination.
4. A solution with lower solute concentration compared to another is called ____________.
A) Isotonic
B) Hypotonic
C) Hypertonic
D) None of these
Answer: B
Explanation: In hypotonic solutions, water moves into cells causing swelling.
5. Plasmolysis occurs when a cell is placed in ____________.
A) Hypotonic solution
B) Isotonic solution
C) Hypertonic solution
D) Distilled water
Answer: C
Explanation: Water moves out of the cell, protoplast shrinks away from cell wall.
6. The pressure responsible for water absorption in roots is ____________.
A) Root pressure
B) Osmotic pressure
C) Turgor pressure
D) Diffusion pressure
Answer: A
Explanation: Root pressure develops due to osmotic uptake of water in xylem.
7. Which instrument is used to study plasmolysis?
A) Simple microscope
B) Compound microscope
C) Electron microscope
D) X-ray diffraction
Answer: B
Explanation: Plasmolysis can be observed in epidermal peel cells under compound microscope.
8. When the cell wall resists further entry of water, the cell is said to be ____________.
A) Flaccid
B) Turgid
C) Plasmolysed
D) Hypertonic
Answer: B
Explanation: Turgid cells exert turgor pressure against the cell wall.
9. Water potential of pure water is ____________.
A) 1
B) 0
C) –1
D) 100
Answer: B
Explanation: Pure water has maximum water potential taken as zero.
10. Active transport in plants requires ____________.
A) ATP energy
B) Osmosis
C) Diffusion
D) Imbibition
Answer: A
Explanation: Active transport moves molecules against concentration gradient using energy.
11. Symplastic movement of water occurs through ____________.
A) Cell wall
B) Cytoplasm via plasmodesmata
C) Vacuole only
D) Xylem vessels only
Answer: B
Explanation: Symplast pathway involves protoplasmic continuum of adjacent cells.
12. Apoplastic movement of water occurs through ____________.
A) Vacuoles
B) Cytoplasm
C) Cell walls and intercellular spaces
D) Plasmodesmata
Answer: C
Explanation: Apoplast is non-living pathway outside plasma membrane.
13. The symplastic pathway is interrupted by ____________.
A) Xylem
B) Phloem
C) Casparian strips
D) Pith
Answer: C
Explanation: Suberized Casparian strips block apoplastic flow in endodermis forcing symplastic entry.
14. The first step in water absorption by roots is ____________.
A) Diffusion
B) Active transport
C) Osmosis
D) Imbibition
Answer: D
Explanation: Root hairs imbibe water before osmotic entry.
15. The term water potential was introduced by ____________.
A) van’t Hoff
B) Slatyer & Taylor
C) Dixon & Joly
D) Strasburger
Answer: B
Explanation: They introduced the concept of water potential in 1960s.
16. When a cell is in isotonic solution, its water potential is ____________.
A) Higher than outside
B) Lower than outside
C) Equal to outside
D) Zero
Answer: C
Explanation: In isotonic solution, no net water movement occurs.
17. Root pressure is maximum in ____________.
A) Dry season
B) Early spring
C) Rainy season
D) Midday summer
Answer: B
Explanation: Root pressure is high in spring when transpiration is low.
18. The swelling of wooden doors in rainy season is due to ____________.
A) Osmosis
B) Imbibition
C) Diffusion
D) Capillarity
Answer: B
Explanation: Wood imbibes water and swells.
19. The units of water potential are ____________.
A) Calories
B) Joules
C) Pascal (pressure units)
D) Litres
Answer: C
Explanation: Water potential is expressed in pressure units (MPa).
20. The diffusion pressure deficit (DPD) is equivalent to ____________.
A) Osmotic pressure – Turgor pressure
B) Osmotic pressure + Turgor pressure
C) Osmotic pressure – Wall pressure
D) Water potential × Turgor pressure
Answer: A
Explanation: DPD = OP – TP; driving force for water absorption.
21. The entry of water into guard cells causing stomatal opening is due to ____________.
A) Active transport of K⁺
B) Passive diffusion of CO₂
C) Plasmolysis
D) Protein synthesis
Answer: A
Explanation: K⁺ influx lowers water potential, water enters guard cells, stomata open.
22. Endosmosis occurs when a cell is placed in ____________.
A) Hypotonic solution
B) Hypertonic solution
C) Isotonic solution
D) None of these
Answer: A
Explanation: Water enters the cell, making it turgid.
23. Exosmosis occurs when a cell is placed in ____________.
A) Hypotonic solution
B) Hypertonic solution
C) Isotonic solution
D) Distilled water
Answer: B
Explanation: Water leaves the cell, leading to plasmolysis.
24. The movement of water from soil into xylem across cortex is mainly ____________.
A) Apoplastic & Symplastic
B) Only active
C) Only through vacuoles
D) By guttation
Answer: A
Explanation: Water moves via apoplast and symplast across root tissues.
25. In higher plants, water mainly moves upward through ____________.
A) Phloem
B) Xylem
C) Cortex
D) Cambium
Answer: B
Explanation: Xylem vessels and tracheids transport water and minerals.
26. The main region of water absorption in roots is ____________.
A) Root cap
B) Meristematic zone
C) Zone of root hairs
D) Zone of elongation
Answer: C
Explanation: Root hairs greatly increase surface area for water absorption.
27. Water absorption by roots is mostly ____________.
A) Passive
B) Active
C) Imbibition
D) Endosmosis
Answer: A
Explanation: Most water uptake occurs due to transpiration pull (a passive process).
28. Root pressure is best demonstrated by ____________.
A) Plasmolysis experiment
B) Guttation
C) Transpiration
D) Phototropism
Answer: B
Explanation: Root pressure pushes water out through hydathodes at leaf tips, seen as guttation drops.
29. The ascent of sap in tall trees is best explained by ____________.
A) Root pressure theory
B) Capillarity theory
C) Cohesion-tension theory
D) Imbibition theory
Answer: C
Explanation: Dixon & Joly proposed that water rises due to transpiration pull and cohesion of water molecules.
30. Capillary theory is insufficient to explain ascent of sap because ____________.
A) Water is not cohesive
B) Trees are tall (up to 100 m)
C) Root pressure is absent
D) It requires ATP
Answer: B
Explanation: Capillary rise cannot explain movement of water beyond a few meters.
31. The cohesion-tension theory was proposed by ____________.
A) Strasburger
B) Dixon & Joly
C) Bose
D) Priestley
Answer: B
Explanation: They proposed this widely accepted theory in 1894.
32. Maximum transpiration occurs through ____________.
A) Lenticels
B) Cuticle
C) Stomata
D) Hydathodes
Answer: C
Explanation: Stomata account for ~90% of total transpiration.
33. The cuticular transpiration is minimum in ____________.
A) Xerophytes
B) Hydrophytes
C) Mesophytes
D) Halophytes
Answer: A
Explanation: Xerophytes have thick cuticles to reduce water loss.
34. The loss of water in liquid form through hydathodes is called ____________.
A) Transpiration
B) Guttation
C) Exudation
D) Dew
Answer: B
Explanation: Root pressure pushes liquid water through hydathodes.
35. Stomata open due to ____________.
A) Loss of turgor in guard cells
B) Gain of turgor in guard cells
C) Photosynthesis in spongy cells
D) Root pressure
Answer: B
Explanation: Turgid guard cells bend, opening stomatal pore.
36. Which factor closes stomata?
A) Blue light
B) Absorption of K⁺ by guard cells
C) Abscisic acid (ABA)
D) Photosynthesis
Answer: C
Explanation: ABA induces stomatal closure during drought stress.
37. The driving force of transpiration pull is ____________.
A) Root pressure
B) Water potential gradient
C) Guttation
D) Osmosis
Answer: B
Explanation: Water moves upward along gradient created by evaporation from leaves.
38. The first step of transpiration is ____________.
A) Movement into xylem
B) Evaporation from mesophyll cells into intercellular spaces
C) Diffusion out through stomata
D) Root pressure
Answer: B
Explanation: Water evaporates from mesophyll into leaf air spaces before diffusing out.
39. The transpiration ratio is ____________.
A) CO₂ fixed / water lost
B) Water lost / CO₂ fixed
C) O₂ released / water lost
D) Water absorbed / water transpired
Answer: B
Explanation: It measures efficiency of water use during photosynthesis.
40. Transpiration is measured by ____________.
A) Potometer
B) Hygrometer
C) Psychrometer
D) Porometer
Answer: A
Explanation: Potometer (Ganong’s potometer) measures water uptake as an indirect measure of transpiration.
41. The least transpiration occurs in ____________.
A) Xerophytes
B) Hydrophytes
C) Mesophytes
D) Halophytes
Answer: B
Explanation: Aquatic plants lose little water due to availability of surrounding water.
42. The phenomenon of guttation occurs mainly at ____________.
A) Stomata
B) Lenticels
C) Hydathodes
D) Cuticle
Answer: C
Explanation: Specialized pores at leaf margins exude water drops due to root pressure.
43. Which plant will show maximum transpiration rate?
A) Cactus
B) Mango
C) Lotus
D) Water hyacinth
Answer: B
Explanation: Mesophytes like mango have broad leaves with many stomata, leading to higher transpiration.
44. During midday in summer, the rate of transpiration ____________.
A) Increases continuously
B) Decreases due to stomatal closure
C) Remains constant
D) Stops completely
Answer: B
Explanation: High temperature + water stress causes stomata to close at midday.
45. Transpiration helps in ____________.
A) Cooling leaves
B) Ascent of sap
C) Distribution of minerals
D) All of these
Answer: D
Explanation: Transpiration aids in cooling, nutrient movement, and water transport.
46. Antitranspirants reduce transpiration by ____________.
A) Increasing photosynthesis
B) Inducing stomatal closure
C) Increasing root pressure
D) Breaking hydrogen bonds
Answer: B
Explanation: Substances like ABA, phenylmercuric acetate reduce transpiration.
47. Water is pulled upward in xylem mainly due to ____________.
A) Root pressure
B) Cohesion and adhesion of water
C) Imbibition
D) Capillarity alone
Answer: B
Explanation: Cohesion-tension mechanism explains long-distance water transport.
48. Which plant shows Crassulacean Acid Metabolism (CAM) to reduce transpiration?
A) Rice
B) Wheat
C) Opuntia
D) Mango
Answer: C
Explanation: CAM plants open stomata at night to minimize water loss.
49. Xerophytes conserve water by ____________.
A) Thick cuticle
B) Sunken stomata
C) Reduced leaf area
D) All of these
Answer: D
Explanation: All are adaptations to reduce transpiration in dry habitats.
50. The ascent of sap stops when ____________.
A) Root pressure is low
B) Cohesion of water is lost (air embolism)
C) Temperature is high
D) Cuticle is thick
Answer: B
Explanation: Air bubbles in xylem break the water column continuity, stopping sap ascent.
51. The main conducting tissue for mineral nutrients in plants is ____________.
A) Phloem
B) Xylem
C) Cortex
D) Cambium
Answer: B
Explanation: Xylem transports water and minerals absorbed from the soil.
52. Essential elements are those which ____________.
A) Are required in large amounts only
B) Cannot be replaced by other elements
C) May or may not be required for metabolism
D) Are required only in hydroponics
Answer: B
Explanation: An element is considered essential if its absence prevents plant growth and no other element can replace it.
53. Hydroponics is a technique used to ____________.
A) Study root nodules
B) Grow plants without soil
C) Prevent transpiration
D) Increase guttation
Answer: B
Explanation: Hydroponics (solution culture) is used to study mineral requirements of plants.
54. Which mineral is part of chlorophyll?
A) Calcium
B) Magnesium
C) Iron
D) Sulphur
Answer: B
Explanation: Magnesium forms the central atom of chlorophyll molecules.
55. Which mineral is essential for nitrogen fixation in legumes?
A) Boron
B) Iron & Molybdenum
C) Potassium
D) Calcium
Answer: B
Explanation: Both are cofactors of nitrogenase enzyme involved in biological nitrogen fixation.
56. Necrosis (death of tissues) is mainly due to deficiency of ____________.
A) K, Ca, Mg
B) Fe, Mn, Zn
C) Mo, B
D) Na, Cl
Answer: A
Explanation: Deficiency of these minerals leads to death of cells and tissues in leaves.
57. Chlorosis (yellowing of leaves) is caused by deficiency of ____________.
A) N, K, Mg, Fe, S, Mo
B) Ca, B, Mn
C) Na, Cl, Zn
D) P, Cu, Mn
Answer: A
Explanation: These minerals are required for chlorophyll formation; deficiency causes chlorosis.
58. Which mineral is needed for photolysis of water during photosynthesis?
A) Fe
B) Mn
C) Mg
D) Ca
Answer: B
Explanation: Manganese ions are essential for water-splitting complex in PSII.
59. Which mineral is most immobile in plants?
A) Nitrogen
B) Phosphorus
C) Calcium
D) Potassium
Answer: C
Explanation: Calcium is not translocated easily, hence deficiency symptoms appear in young leaves first.
60. Which mineral is required for synthesis of auxin (IAA)?
A) Fe
B) Zn
C) Cu
D) Mg
Answer: B
Explanation: Zinc is a cofactor for enzymes involved in tryptophan to IAA conversion.
61. The main tissue for transport of organic food in plants is ____________.
A) Xylem
B) Phloem
C) Cambium
D) Endodermis
Answer: B
Explanation: Phloem transports sucrose and other organic solutes.
62. Food is transported in phloem mainly in the form of ____________.
A) Glucose
B) Fructose
C) Sucrose
D) Starch
Answer: C
Explanation: Sucrose is the common transport sugar in most plants.
63. In phloem, which cells are responsible for transport?
A) Companion cells & sieve tube elements
B) Tracheids & vessels
C) Phloem parenchyma
D) Cambium cells
Answer: A
Explanation: Sieve tubes transport food, and companion cells help in loading/unloading.
64. The direction of food transport in phloem is ____________.
A) Only upward
B) Only downward
C) Both upward and downward
D) Random
Answer: C
Explanation: Food is transported bidirectionally depending on source-sink relationship.
65. The pressure-flow hypothesis was proposed by ____________.
A) Dixon & Joly
B) Münch
C) Strasburger
D) Priestley
Answer: B
Explanation: In 1930, Münch proposed the mass flow or pressure-flow hypothesis for phloem transport.
66. According to pressure-flow hypothesis, transport in phloem occurs due to ____________.
A) Root pressure
B) Turgor pressure gradient
C) ATP energy only
D) Cohesion-tension
Answer: B
Explanation: Osmotic pressure builds in source region, creating a pressure difference moving food to sink.
67. In phloem loading, sugars move from mesophyll cells into sieve tubes by ____________.
A) Passive diffusion
B) Active transport
C) Osmosis
D) Capillarity
Answer: B
Explanation: Energy (ATP) is required to load sucrose against concentration gradient into sieve tubes.
68. In pressure-flow mechanism, “source” refers to ____________.
A) Organs where food is utilized
B) Organs where food is stored
C) Organs where food is synthesized
D) Root apex
Answer: C
Explanation: Source = photosynthetic leaves that produce sugars.
69. In pressure-flow mechanism, “sink” refers to ____________.
A) Leaves
B) Roots, fruits, seeds
C) Xylem
D) Cambium
Answer: B
Explanation: Sink = tissues where sugars are stored or consumed.
70. Phloem transport is ____________.
A) Apoplastic
B) Symplastic
C) Both apoplastic and symplastic
D) Vacuolar only
Answer: C
Explanation: Sugars move by symplast (plasmodesmata) and apoplast (active loading into phloem).
71. Translocation of food in phloem requires ____________.
A) Light
B) ATP
C) Oxygen
D) Both B and C
Answer: D
Explanation: ATP and aerobic respiration provide energy for phloem loading/unloading.
72. The translocation rate in phloem is ____________.
A) 1 mm/hour
B) 1 m/hour
C) 1 cm/day
D) 0.1 mm/day
Answer: B
Explanation: Food moves rapidly in phloem compared to diffusion.
73. The girdling experiment (removal of phloem) shows that ____________.
A) Water transport occurs in phloem
B) Food transport occurs in phloem
C) Minerals move in cortex
D) Transpiration pull occurs in phloem
Answer: B
Explanation: Swelling above girdled area proves food is transported through phloem.
74. The sieve plates in phloem are present in ____________.
A) Sieve tube elements
B) Companion cells
C) Xylem tracheids
D) Phloem parenchyma
Answer: A
Explanation: Sieve plates are perforated walls of sieve tubes facilitating transport.
75. The energy for phloem transport comes from ____________.
A) Photosynthesis directly
B) Active loading by companion cells
C) Transpiration pull
D) Root pressure
Answer: B
Explanation: Companion cells use ATP to load sugars into sieve tubes, generating pressure flow.
76. The cohesion-tension theory of water transport explains ____________.
A) Phloem transport
B) Ascent of sap in xylem
C) Mineral nutrition
D) Osmosis in root hairs
Answer: B
Explanation: Cohesion-tension theory (Dixon & Joly) explains upward pull of water due to transpiration.
77. Root pressure alone cannot account for water rise in tall trees because ____________.
A) It is too weak
B) It occurs only at night
C) It is absent in dry soil
D) All of these
Answer: D
Explanation: Root pressure is weak and insufficient to explain ascent of sap beyond a few meters.
78. Which condition favours guttation?
A) Low root pressure, high transpiration
B) High root pressure, low transpiration
C) High transpiration, high temperature
D) None of these
Answer: B
Explanation: Guttation occurs at night/early morning when transpiration is low but root pressure is high.
79. Transpiration pull is strongest in ____________.
A) Midday
B) Midnight
C) Early morning
D) Evening
Answer: A
Explanation: High temperature and low humidity at midday cause maximum transpiration pull.
80. Which experiment proves that food is translocated through phloem?
A) Plasmolysis experiment
B) Girdling experiment
C) Potometer experiment
D) Bell jar experiment
Answer: B
Explanation: Removing a ring of bark (phloem) causes accumulation of food above the girdle.
81. Food translocation in plants is always ____________.
A) From root to shoot
B) From leaves to roots only
C) From source to sink
D) Random
Answer: C
Explanation: Food transport direction depends on where it is produced (source) and where it is needed (sink).
82. The rate of translocation in phloem is approximately ____________.
A) 1 cm/hr
B) 1 m/hr
C) 1 cm/day
D) 1 mm/hr
Answer: B
Explanation: Phloem transport is rapid compared to diffusion.
83. Which plant shows CAM photosynthesis to conserve water?
A) Wheat
B) Rice
C) Opuntia
D) Mango
Answer: C
Explanation: CAM plants open stomata at night, reducing water loss.
84. The transpiration-cohesion theory fails if ____________.
A) Water molecules lose cohesion (embolism)
B) Transpiration rate decreases
C) Light intensity is low
D) Root pressure develops
Answer: A
Explanation: Air bubbles in xylem break water column, stopping ascent of sap.
85. In hydrophytes, cuticle and transpiration are ____________.
A) Thick and high
B) Absent and negligible
C) Thick and negligible
D) Thin and high
Answer: B
Explanation: Aquatic plants don’t need thick cuticle as water loss is not an issue.
86. Xerophytes minimize water loss by ____________.
A) Thick cuticle
B) Sunken stomata
C) Reduced leaves (spines)
D) All of these
Answer: D
Explanation: These are common xerophytic adaptations.
87. The movement of sugars from mesophyll cells to phloem is called ____________.
A) Phloem loading
B) Phloem unloading
C) Transpiration pull
D) Mineral transport
Answer: A
Explanation: Sugars are actively loaded into sieve tubes from source cells.
88. Water absorption through mycorrhizal association is most important for ____________.
A) Nitrogen
B) Potassium
C) Phosphorus
D) Calcium
Answer: C
Explanation: Mycorrhiza greatly enhances phosphorus uptake.
89. Which ion plays a major role in stomatal movement?
A) Na⁺
B) K⁺
C) Ca²⁺
D) Mg²⁺
Answer: B
Explanation: Active uptake of K⁺ by guard cells regulates stomatal opening/closure.
90. During drought stress, stomata close due to ____________.
A) Auxin
B) Abscisic acid (ABA)
C) Cytokinin
D) Ethylene
Answer: B
Explanation: ABA induces stomatal closure to conserve water.
91. In phloem, the living cells that assist sieve tubes are ____________.
A) Phloem fibres
B) Phloem parenchyma
C) Companion cells
D) Cambium cells
Answer: C
Explanation: Companion cells help in loading/unloading of sugars into sieve tubes.
92. In tall trees, ascent of sap occurs mainly due to ____________.
A) Root pressure
B) Transpiration pull
C) Capillarity
D) Osmosis
Answer: B
Explanation: Root pressure is insufficient; cohesion-tension mechanism explains water rise.
93. Which plant factor reduces transpiration?
A) Large leaf area
B) Sunken stomata
C) Thin cuticle
D) High stomatal frequency
Answer: B
Explanation: Sunken stomata reduce water loss by trapping humid air.
94. In girdling experiment, food accumulates ____________.
A) Below the girdle
B) Above the girdle
C) Both above and below
D) At the root tip
Answer: B
Explanation: Phloem is removed, so sugars cannot move downward.
95. Which factor reduces the rate of transpiration?
A) High temperature
B) Low humidity
C) High wind velocity
D) High atmospheric humidity
Answer: D
Explanation: When air is already saturated with water, transpiration rate decreases.
96. The process of guttation is mainly seen in ____________.
A) Tall trees
B) Herbs with large leaves
C) Succulents
D) Xerophytes
Answer: B
Explanation: Guttation occurs through hydathodes, commonly in plants like tomato, colocasia, grasses.
97. Active transport in phloem requires ____________.
A) ATP
B) Osmosis
C) Root pressure
D) Cohesion
Answer: A
Explanation: Loading and unloading of sugars in phloem requires metabolic energy.
98. The closing of stomata at night is mainly due to ____________.
A) CO₂ accumulation
B) Loss of turgor in guard cells
C) High transpiration rate
D) ABA action
Answer: B
Explanation: Guard cells lose water at night → stomata close.
99. Which statement is correct regarding translocation of food?
A) It occurs only in downward direction
B) It occurs only during the day
C) It occurs both upward and downward
D) It is independent of source and sink
Answer: C
Explanation: Phloem translocation is bidirectional (source to sink).
100. Which is the most accepted theory of food transport in plants?
A) Diffusion theory
B) Protoplasmic streaming theory
C) Pressure-flow hypothesis
D) Electro-osmotic theory
Answer: C
Explanation: Münch’s pressure-flow (mass flow) hypothesis is widely accepted for phloem translocation.
